1. Chiyambi
Makina onse a Viral Natic Nucle Exprect Tot Kit ndi yoyenera kuti ichotsere ma acid onse ochokera ku seramu, plasma, minofu homogenate ndi zitsanzo zina. Kit idakhazikitsidwa paukadaulo wa silika woyeretsera, zomwe zimathetsa kufunika kwa poizol phenol-chloroform yowonjezera ndi nthawi yomwa mowa. Izi zathetsa bwino ma acid a acid a hepatitis ba / c, hepatitis C, ndi Norwarrup Standard. Kulandila DNA / RNA ikhoza kugwiritsidwa ntchito mwachindunji pamayeso angapo opita pansi monga PCR, RT-PCR, ndi nyali.
Zindikirani:
1. Wonyamula RNA yolimba iyenera kusungunuka m'madzi aulere aulere ku 1μg / μl musanagwiritse ntchito, ndipo vortex kuti isungunuke. Sungani mu Alliquots ku -70 ° C. Ngati mukufuna kusunga pa -20 ℃ kwa nthawi yayitali, chonde lembani molingana ndi kuchuluka kwa nthawi yogwiritsa ntchito.
2. Sungunulani protenase k (20mg / ml): Onjezani proteanase shuleve Buffer kuti asungunuke protenase k mpaka ponseponse wa 20mg / ml. Proteanase K owuma ufa akhoza kusungidwa pa 2-8 ° C kwa chaka chimodzi, koma proteinsed proteanase k uyenera kusungidwa mu Alliquots ku -20 c. Kubwereza mobwerezabwereza ndi kuwononga mapuloteni K kungakhudze zochita zake.
3. Buffer Vhb iyenera kuchepetsedwa ndi 14 ml MthethelEl musanagwiritse ntchito ndikusungidwa kutentha.
4. Buffer RW2 iyenera kuchepetsedwa ndi 80 ml ya 80 ml yonse mtheradi asanagwiritse ntchito ndikusungidwa kutentha.
3. Alumali moyo
Kupatula Proteanase K ndi Carrier RNA, zina za mankhwalawa zitha kusungidwa kutentha (15-25 ° C) kwa miyezi 12, ndipo iyenera kusungidwa pa 2-8 ° C posungira nthawi yayitali. Proteanase K ndi chonyamula RNA wouma ufa umatengedwa kutentha. Chonde sitolo ya -20 ° C mutalandira mayeso, ndipo malo ogulitsira 30 ° C mutatha kusungunuka.
